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Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 4 (2020) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2020.904.060 Effect of Soil and Foliar Application of Zinc on Growth and Yield of Greengram (Vigna radiate L.) H. V. Vinodkumar, S. Channakeshava*, B. Basavaraja and Ananathakumar College of Agriculture, V.C Farm Mandya, University of Agricultural Sciences, Bengaluru-560065, Karnataka, India *Corresponding author ABSTRACT Keywords Zinc sulphate, Zn EDTA, Foliar spray Article Info Accepted: 07 March 2020 Available Online: 10 April 2020 A field experiment was conducted to study the Response of greengram to soil and foliar application of zinc in southern dry zone of Karnataka during early kharif 2018 at Junjanahally, Hassan. Experiments consist of nine treatments replicated thrice with RCBD. Results revealed that significantly higher growth and yield parameters such as plant height(71.93 cm) number of branches per plant(4.37), number of effective nodules per plant(28.07) dry matter production per plant (27.07g), pod length (13.60 cm) number of pods per plant(24.40), number of seeds per pod (13.40), pod weight per plant (29.73g),seed yield (1022.99kg/ha) and haulm yield (2544.93kg/ha) were recorded with T9 (soil application of zinc sulphate @ 10 kg/ha + foliar spray of zinc EDTA at 1.0 percent along with NPK and FYM). The available nitrogen, phosphorus and potassium content of soil were non significant however higher nitrogen and potassium recorded (239.80& 177.10 kg/ha) in the treatment T1(NPK+FYM) and lower nitrogen and potassium recorded (232.70 &171.40 kg/ha)in T9 (T3 + FSZn EDTA @1.0%). Higher phosphorus recorded in T9 (44.70 kg/ha). As regard to DTPA extractable micronutrients in soil , low iron (7.69 mg/kg),copper (0.369ppm),manganese (8.16 ppm) and Boron (0.35 mg/kg) content in soil was recorded in T9 (T3+ FS of Zn EDTA).Application of zinc increased carbonate bound zinc (3.14mg/kg), , organic bound zinc (1.73 mg/kg) and Fe and Al oxide bound zinc (2.84 mg/kg) was recorded in T3 (T1+ SA of ZnSO 4 @10 kg/ha) over with NPK+ FYM. The higher total zinc was observed in the treatment T9 (130.42 mg/kg) which received T3+ FS of Zn EDTA@1.0 percent followed by treatment T7(135.32 mg/kg) and lowest Zn content was recorded (130.42 mg/kg) in the treatment T1(NPK+FYM). importance sources of protein, vitamin and minerals. Important pulses which grown in India are greengram, chickpea pigeon pea horse gram, cowpea and rice bean. It is asserted that in India pulse are cultivated on an area of 29.36 mha with a production and productivity of 24.51 mt and 835 kg per hectare (Anon., 2018). Low productivity of of Introduction Pulses play a pivotal role in nutritional security. considering this Food and Agriculture Organization (FAO) has marked year 2016 as international year of pulses to show-cause the importance of pulses in human and animal nutrition. Pulses are 501 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 these pulses ascribed to the conditions under which these are grown. In general pulses grown in rainfed area in marginal soils under residual moisture conditions and most importantly without or merger fertilizer input. Materials and Methods A field experiment was conducted during early Kharif 2018 to study the “Response of greengram (Vigna radiata L.) to soil and foliar application of zinc in Southern Dry Zone of Karnataka” at farmers field, Junjanahalli, Hassan, Karnataka. The soil was sandy loam in texture with 78.10, 4.90 and 17.00 per cent sand, silt and clay, respectively. The soil was slightly alkaline inpH (8.0) reaction and normal in soluble salts (0.24 dS m-1). The soil was low in organic carbon (4.8 g kg-1) content and medium in available nitrogen (298 kg ha-1), available P2O5 (38.5 kg ha-1), available K2O (189 kg ha-1) and low in sulphur (9.1mg kg-1). Exchangeable calcium and magnesium content of soil was 3.6 and 1.7 cmol kg-1, respectively. The soil selected was deficient in zinc with zinc content of 0.52 mg kg-1 and the DTPA extractable micronutrient content viz., iron, copper, manganese and hot water soluble boron were 8.65, 0.42, 8.93 and 0.38 mg kg-1, respectively. Experiment was laid out during early Kharif 2018 in Randomized Complete Block Design. There are nine treatments replicated thrice. The size of each plot was 5.1 m x 2 m (gross). Treatments were distributed randomly in the plots within the blocks (Table 1). Green gram known as Mung or Golden gram is native to Indian continent. Apart from India it is also grown in China, Thailand, Indonesia, Burma, Bangladesh, Laos and Cambodia and also in hot and dry regions of southern Europe and southern united states. The favourable conditions for its better growth and development are warm humid climate with an optimum temperature range of 250 to 35 0 C with moderate rains of 80-100 cm. Normally it is sown during April to June as high temperature and low humidity keeps insects and diseases infestations at below threshold level(Anon,2017a). In India it is grown on an area of 43.05 lakh hectare with annual production of 20.70 lakh tonne and productivity of 481 kgper hectare (Anno., 2017b). Greengram contains 25 percent protein, 1.3 percent fat and 57 percent carbohydrate. Green gram is also rich source of calcium (68 mg/100gseed), phosphorus (300g/100g seed) and Iron (7 mg/ 100 gseed). It is one of the main source of protein and certain essential amino acids like tryptophan and lysine in vegetarian diets. It also a rich source of energy and provides 334 kilocalories of energy (Srivastava and Ali, 2004). Wide spread deficiency of micronutrient has become major constraint for achieving higher genetic potential yield of greengram along with quality of crop. Among the micronutrients the deficiency of Zinc and boron has become wide spread in recent years all over the country. In India next to Nitrogen, phosphorus, potassium and sulphur 5th important yield limiting nutrient is zinc which accounts for 48 percent of Indian soils. Hence, an investigation entitled “Response of greengram (Vigna radiata L.) to soil and foliar application of zinc in Southern Dry Zone of Karnataka” was carried out The treatment details of the research are as follows: T1: NPK + FYM T2: T1+ SA of ZnSO4. 7H2O @ 5 kg ha-1 T3: T1+ SA of ZnSO4. 7H2O @ 10 kg ha-1 T4: T1+ FS of Zn EDTA @ 0.50 % T5: T1+ FS of Zn EDTA @ 1.00 % T6: T2+ FS of Zn EDTA @ 0.50 % T7: T3+ FS of Zn EDTA @ 0.50 % T8: T2+ FSof Zn EDTA @ 1.00 % T9: T3+ FS of Zn EDTA @ 1.00 % NPK: 12.5:25:25 N: P2O5:K2Okg ha-1.FYM: Farm yard manure. SA: Soil application. FS: Foliar spray at 35 DAS. 502 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 Zn EDTA Results and Discussion Zinc Ethylene diamine tetra acetic acid. Greengram variety BGS-9 was grown as a test crop. Results of experiment revealed that the growth parameters like plant height (70.27 cm) and drymatter production (27.07g) was higher in treatments receiving soil application of zinc sulphate @ 10 kg per hectare and foliar application of 1% Zn EDTA along with NPK+ FYM which was on par with T3(NPK+FYM+SA of Zn and FS of ZnEDTA0.5%). Lower plant height (60.73cm) and dry matter production was recorded in T1(NPK+FYM applied). Fertilizer application The calculated quantity of urea, diammonium phosphate (DAP) and murate of potash (MOP) i.e. 5.89: 54.34: 41.66 kg ha-1, respectively was used to supply N, P, K respectively as per the treatments. The fertilizers were applied at the time of sowing as basal. The higher plant height and dry matter production was attributed to sufficient supply of zinc through foliar spray and soil application which influenced on growth and development of plant. Similar results were also recorded by Akay (2011). All the fertilizers were applied in line opened 4 cm away from the seed row and mixed well with soil. Soil application of zinc was took at the time of sowing and foliar spray of zinc EDTA at 35 DAS. Greengram seeds were inoculated with rhizobium inoculum before sowing and sown with spacing of 30 cm X 10 cm. Significantly higher pod length(13.60 cm), number of pods plant-1 (24.40), number of seeds pod-1(13.40), pod weight per plant1 (29.73 g) and test weight (5.58g) were obtained in treatment T9 with soil application of zinc sulphate at 10 kg ha-1+ foliar spray of zinc EDTA at 1.00 % along with NPK and FYM. Lower pod length (11.53cm), number of pods per plant, pod weight and test weight recorded in T1 (NPK + FYM). Biometric observations on growth parameters like plant height, number of leaves per plant, leaf area, dry matter production, Number of nodules and effective nodules. Yield attributing parameters like number of pods per plant, number of seeds per plant, pod length, pod weight and test weight were recorded. Soil and plant samples were analysed for major (NPK) and micronutrient (Cu Zn Fe Mn) using standard procedure prescribed by Jackson (1973) and Lindsay and Norwel (1978). The positive effect of zinc on growth character might be due to its direct influence on synthesis of tryptophan, a precursor for production of growth hormone i.e. auxin. Auxin has key role in cell elongation process which in turn helps in plant growth and development (Krishna, 1995) (Table 2 and 3). Estimation of total zinc in soils is described by Sridhar and Jackson (1974). The concentration of zinc in the above solutions was determined by atomic absorption spectrophotometer under suitable measuring conditions (Page et al., 1982). Estimation of Fractions of zinc in soils defined as given below (Ma and Uren, 1995). Seed yield (kg ha-1) Significantly higher seed yield was obtained with the application ofT9 (T3+ FS of Zn EDTA @ 1.00 %) (1022.19 kg ha-1) which was significantly superior over the treatments 503 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 T1 (805.51kg ha-1), T2 (938.14 kg ha-1), (879.51 kg ha-1), T5 (905.84 kg ha-1) and (943.89 kg ha-1) and it was on par with (982.65 kg ha-1), T8 (968.50 kg ha-1), and (973.00 kg ha-1). T4 T6 T7 T3 The data on DTPA extractable Fe, Mn, Zn, Cu and hot water soluble B status (g ha-1) of soil at harvest of crop as influenced by soil and foliar application of zinc are presented as below. Haulm yield (kg ha-1) Iron The effect of soil and foliar application of zinc on haulm yield is presented in Table 4. Significantly higher haulm yield was obtained with the application of T9 (T3+ FS of Zn EDTA @ 1.00 %) (2544.93 kg ha-1) which was on par with T3 (2453.43 kg ha-1), T6 (2417.00 kg ha-1), T7 (2458.57 kg ha-1) and T8 (2442.39 kg ha-1) and was significantly superior over the treatments T1 (2217.80 kg ha-1), T2 (2402.26 kg ha-1), T4 (2387.88 kg ha-1) and T5 (2390.27 kg ha-1). The iron content did not vary significantly among the treatments. However, higher iron content was observed in the treatment T1 (7.90 mg kg-1) which received NPK along with FYM, followed by T4 (7.87 mg kg-1). The lowest was in T9 (T3+ FS of Zn EDTA @ 1.00 %) (7.69 mg kg-1). Manganese The manganese content did not vary significantly among the treatments. However, higher manganese content was observed in the treatment T1 (8.27 mg kg-1) which received NPK along with FYM, followed by T5 and T7 (8.25 mg kg-1). The lowest was in T9 (T3+ FS of Zn EDTA @ 1.00 %)(8.16 mg kg-1). The adequate availability of zinc in soil results in growth parameters, photosynthesis efficiency, assimilation and production (Ali, 2004) and in turn to achieve higher yield and yield attributes in basal or combined application treatments i.e. T9 (T3+ FS of Zn EDTA @ 1.00 %), T7 (T3+ FS of Zn EDTA @ 0.50 %), T8 (T2+ FS of Zn EDTA @ 1.00 %), T6 (T2+ FS of Zn EDTA @ 0.50 %), T3 (T1+ SA of ZnSO4 7H2O @ 10 kg ha-1) and T2 (T1+ SA of ZnSO4 7H2O @ 5 kg ha-1). The findings are also in agreement with the verdicts of Yashona et al., (2018). Zinc The data in Table 6 indicated that DTPA extractable Zn content in soil differed significantly due to treatments. Higher zinc content in soil was recorded in T9 (0.90 mg kg-1) which receivedT3+ FS of Zn EDTA @ 1.00 per cent which was on par with T7and T3 which were 0.88 mg kg-1 and 0.87 mg kg1 respectively and was significantly higher than Treatment T1 i.e, NPK + FYM (0.46 mg kg-1) and rest of the treatments (T2, T4, T5, T6 and T8). The available Nitrogen, phosphorous and potassium content of the soil after harvest was non-significant. However the lower Nitrogen and potassium content were found in treatment T9 (T3 + FS of Zn EDTA @ 1.00 %) which were 232.70 kg ha-1 and 171.40 kg ha-1 respectively and higher N and K2O content were found in treatment T1 (NPK+FYM) which were 239.80 kg ha-1and 177.10kg ha-1. Higher P2O5content was found in treatment T9 (44.70 kg ha-1) and lowest was recorded in treatment T1 (41.60 kg ha-1) (Table 5). The data on DTPA extractable Fe, Mn, Zn, Cu and hot water soluble B status (g ha-1) of soil at harvest of crop as influenced by soil and foliar application of zinc are presented in the Table 6 and Table 8. 504 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 EDTA @ 1.00 per cent which was on par with T7and T3 which were 0.88 mg kg-1 and 0.87 mg kg-1respectively and was significantly higher than Treatment T1i.e, NPK + FYM (0.46 mg kg-1) and rest of the treatments (T2, T4, T5, T6 and T8). Iron The iron content did not vary significantly among the treatments. However, higher iron content was observed in the treatment T1 (7.90 mg kg-1) which received NPK along with FYM, followed by T4 (7.87 mg kg-1). The lowest was in T9 (T3+ FS of Zn EDTA @ 1.00 %) (7.69 mg kg-1). Significantly higher zinc content in soil was recorded in T9 (T3+ FS of Zn EDTA @ 1.00 %). The results are in line with the verdicts of Sharma et al., (2016). The increase in zinc content might be due to the considerable exchange of Zinc on the clay complexes with other cations on its addition. The results are in accordance with the findings of Kulandaivel et al., (2004). Manganese The manganese content did not vary significantly among the treatments. However, higher manganese content was observed in the treatment T1 (8.27 mg kg-1) which received NPK along with FYM, followed by T5 and T7 (8.25 mg kg-1). The lowest was in T9 (T3+ FS of Zn EDTA @ 1.00 %)(8.16 mg kg-1). Copper The treatments had non-significant effect on copper status in soil. However, higher copper content was recorded in T1 (NPK + FYM) with 0.392 mg kg-1followed by treatment T4 (0.389 mg kg-1) and lowest copper content was observed in treatment T9 (T3 + FS of Zn EDTA @ 1.00 %) which was 0.369 mg kg-1 (Table 7). Zinc The DTPA extractable Zn content in soil differed significantly due to treatments. Higher zinc content in soil was recorded in T9 (0.90 mg kg-1) which receivedT3 + FS of Zn Table.1 Fractions of soil at the experimental site Sl. No Fraction Value (mg kg-1) 1. Water soluble zinc 0.37 2. Sorbed zinc 2.10 3. Easily reducible manganese bound zinc 3.71 4. Carbonate bound zinc 2.73 5. Organic bound zinc 1.04 6. Fe & Al oxides bound zinc 2.44 7. Residual zinc 122.19 8. Total zinc 134.58 505 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 Table.2 Effect of soil and foliar application of zinc on growth parameters of greengram Treatments T1: NPK + FYM T2: T1+ SA of ZnSO47H2O @ 5 kg ha-1 T3: T1+ SA of ZnSO47H2O @ 10 kg ha-1 T4: T1+ FS of Zn EDTA @ 0.50 % T5: T1+ FS of Zn EDTA @ 1.00 % T6: T2+ FS of Zn EDTA @ 0.50 % T7: T3+ FS of Zn EDTA @ 0.50 % T8: T2+ FS of Zn EDTA @ 1.00 % T9: T3+ FSof Zn EDTA @ 1.00 % S.Em± CD (P=0.05) Growth Parameters Plant height Drymatter Number of (cm) production (g) branches/plant 60.73 20.15 3.47 65.63 23.08 3.73 68.93 26.02 4.20 63.17 21.61 3.53 64.40 22.00 3.60 66.43 24.10 3.83 70.27 26.44 4.30 66.67 25.23 3.90 71.93 27.07 4.37 1.52 4.55 0.98 2.93 0.13 0.40 Table.3 Effect of soil and foliar application of zinc on yield and yield parameters of greengram Treatments T1: NPK + FYM T2: T1+ SA of ZnSO47H2O @ 5 kg ha-1 T3: T1+ SA of ZnSO47H2O @ 10 kg ha-1 T4: T1+ FS of Zn EDTA @ 0.50 % T5: T1+ FS of Zn EDTA @ 1.00 % T6: T2+ FS of Zn EDTA @ 0.50 % T7: T3+ FS of Zn EDTA @ 0.50 % T8: T2+ FS of Zn EDTA @ 1.00 % T9: T3+ FSof Zn EDTA @ 1.00 % S.Em± CD (P=0.05) Pod length (cm) 11.53 12.80 No. of pods plant-1 19.27 22.13 No. of seeds pod-1 11.80 12.80 Pod weight(g) 23.33 25.68 Test weight (g) 4.89 5.19 13.17 23.40 13.13 27.89 5.63 12.43 12.60 13.00 13.30 13.13 13.60 0.30 0.91 19.73 20.67 22.93 24.07 23.07 24.40 0.62 1.86 12.13 12.60 12.87 13.33 12.93 13.40 0.27 0.80 24.92 25.07 25.94 28.85 26.89 29.73 0.76 2.27 5.12 5.16 5.33 5.58 5.39 5.58 0.15 NS 506 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 Table.4 Effect of soil and foliar application of zinc on seed, haulm yield and harvest Index of greengram Treatments Seed Yield (kg/ha) Haulm yield Harvest Index (%) (kg/ha) 805.51 2217.80 0.267 938.14 2402.26 0.281 973.00 2453.43 0.284 T4: T1+ FS of Zn EDTA @ 0.50 % 879.51 2387.88 0.269 T5: T1+ FS of Zn EDTA @ 1.00 % 905.84 2390.27 0.275 T6: T2+ FS of Zn EDTA @ 0.50 % 943.89 2417.00 0.281 T7: T3+ FS of Zn EDTA @ 0.50 % 982.65 2458.57 0.285 T8: T2+ FS of Zn EDTA @ 1.00 % 968.50 2442.39 0.284 T9: T3+ FSof Zn EDTA @ 1.00 % 1022.19 2544.93 0.287 S.Em± 24.62 43.06 0.006 CD (P=0.05) 73.81 129.09 NS T1: NPK + FYM T2: T1+ SA of ZnSO47H2O @ 5 kg ha -1 T3: T1+ SA of ZnSO47H2O @ 10 kg ha -1 Table.5 Available N, P2O5 and K2O content of soil after harvest of greengram as influenced by soil and foliar application of zinc Treatment N P2O5 -1 K2O (kg ha ) (kg ha ) (kg ha-1) T1: NPK + FYM 239.80 41.60 177.10 T2: T1+ SA of ZnSO47H2O @ 5 kg ha-1 236.62 42.10 174.23 T3: T1+ SA of ZnSO47H2O @ 10 kg ha-1 234.60 43.80 171.60 T4: T1+ FS of Zn EDTA @ 0.50 % 239.64 42.96 176.14 T5: T1+ FS of Zn EDTA @ 1.00 % 237.50 43.80 175.55 T6: T2+ FS of Zn EDTA @ 0.50 % 237.20 44.30 173.30 T7: T3+ FS of Zn EDTA @ 0.50 % 234.80 42.80 172.80 T8: T2 + FS of Zn EDTA @ 1.00 % 238.90 43.90 174.90 T9: T3+ FS of Zn EDTA @ 1.00 % 232.70 44.70 171.40 S.Em± 6.27 1.61 5.21 CD (P=0.05) NS NS NS SA: Soil application FS: Foliar spray at 35 DAS 507 -1 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 Table.6 DTPA extractable iron, manganese and zinc content of soil after harvest of greengram as influenced by soil and foliar application of zinc Fe(mg kg-1) Mn(mg kg-1) Zn(mg kg-1) T1: NPK + FYM 7.90 8.27 0.46 T2: T1+ SA of ZnSO47H2O @ 5 kg ha-1 7.79 8.21 0.79 T3: T1+ SA of ZnSO47H2O @ 10 kg ha-1 7.76 8.19 0.87 T4: T1+ FS of Zn EDTA @ 0.50 % 7.87 8.24 0.48 T5: T1+ FS of Zn EDTA @ 1.00 % 7.81 8.25 0.50 T6: T2+ FS of Zn EDTA @ 0.50 % 7.79 8.22 0.81 T7: T3+ FS of Zn EDTA @ 0.50 % 7.72 8.25 0.88 T8: T2 + FS of Zn EDTA @ 1.00 % 7.70 8.21 0.82 T9: T3+ FS of Zn EDTA @ 1.00 % 7.69 8.16 0.90 S.Em± 0.23 0.25 0.02 CD (P=0.05) NS NS 0.07 Treatment NOTE: SA: Soil application FS: Foliar spray at 35 DAS Table.7 DTPA extractable copper and hot water soluble boron content of soil Cu (mg kg-1) B (mg kg-1) T1: NPK + FYM 0.392 0.35 T2: T1+ SA of ZnSO4 7H2O @ 5 kg ha-1 0.386 0.34 T3: T1+ SA of ZnSO47H2O @ 10 kg ha-1 0.381 0.32 T4: T1+ FS of Zn EDTA @ 0.50 % 0.389 0.33 T5: T1+ FS of Zn EDTA @ 1.00 % 0.381 0.33 T6: T2+ FS of Zn EDTA @ 0.50 % 0.383 0.33 T7: T3+ FS of Zn EDTA @ 0.50 % 0.378 0.32 T8: T2 + FS of Zn EDTA @ 1.00 % 0.373 0.33 T9: T3+ FS of Zn EDTA @ 1.00 % 0.369 0.31 S.Em± 0.012 0.01 NS NS Treatment CD (P=0.05) 508 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 Table.8 Distribution of zinc fractions (mg kg -1) in soil at harvest of greengram as influenced by soil and foliar application of zinc Treatment WS-Zn SORB-Zn ERMn-Zn CA-Zn OM-Zn Fe & Alox-Zn RES-Zn Total-Zn T1 0.32 (0.25) 1.87 (1.43) 3.45 (2.65) 2.31 (1.77) 0.96 (0.74) 2.27 (1.74) 119.24 (91.43) 130.42 T2 0.40 (0.30) 3.79 (2.82) 4.08 (3.03) 2.89 (2.15) 1.61 (1.20) 2.68 (1.99) 119.15 (88.52) 134.60 T3 0.45 (0.33) 3.86 (2.86) 4.14 (3.06) 3.14 (2.32) 1.73 (1.28) 2.84 (2.10) 119.03 (88.05) 135.19 T4 0.33 (0.25) 1.79 (1.37) 3.43 (2.62) 2.33 (1.78) 0.93 (0.71) 2.32 (1.77) 119.79 (91.50) 130.92 T5 0.33 (0.25) 1.84 (1.41) 3.47 (2.66) 2.34 (1.79) 0.91 (0.70) 2.33 (1.79) 119.22 (91.40) 130.44 T6 0.39 (0.29) 3.77 (2.80) 4.10 (3.05) 2.83 (2.10) 1.64 (1.22) 2.72 (2.02) 119.18 (88.52) 134.63 T7 0.46 (0.34) 3.87 (2.86) 4.20 (3.10) 3.06 (2.26) 1.76 (1.30) 2.87 (2.12) 119.10 (88.01) 135.32 T8 0.40 (0.30) 3.76 (2.79) 4.09 (3.04) 2.88 (2.14) 1.62 (1.20) 2.71 (2.01) 119.13 (88.51) 134.59 T9 0.45 (0.33) 3.89 (2.87) 4.18 (3.09) 3.10 (2.29) 1.78 (1.31) 2.89 (2.13) 119.08 (87.97) 135.37 S.Em± 0.01 0.10 0.12 0.08 0.04 0.08 3.64 3.99 CD (P=0.05) 0.04 0.29 0.35 0.25 0.13 0.23 NS NS Figures in parenthesis indicate percentage of total Zn 509 Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 501-512 Fig.1 Distribution of zinc fractions (WS-Zn, SORB-Zn, CA-Zn, OM-Zn, Fe & Alox-Zn) (mg kg -1) in soil at harvest of greengram as influenced bysoil and foliar application of zin WS-Zn SORB-Zn ERMn-Zn CA-Zn OM-Zn Fe & Alox-Zn Zn fractions content (mg kg1) 4.50 4.00 3.50 3.00 2.50 2.00 1.50 1.00 0.50 0.00 T1 T2 T3 T4 T5 T6 T7 T8 T9 Treatments (0.40 mgkg-1), T4 (0.33mgkg-1), T5 (0.33mg kg-1), T6 (0.39 mg kg-1) and T8 (0.40 mg kg-1). Boron Boron content in the soil did not varied significantly due to treatments. However higher B content of 0.35 mg kg-1 was observed in T1(NPK + FYM) and the minimum boron content was recorded in T9 (0.31 mg kg-1) which received T3+ FS of Zn EDTA @ 1.00 %.Total zinc content in soil not differed significantly due to treatment. However, higher total zinc was observed in treatment T9 (135.37 mg kg-1) which received T3+ FS of Zn EDTA @ 1.00 per cent followed by treatment T7 (135.32 mg kg-1) and lowest total zinc was observed in the treatment T1 (NPK+ FYM) which was 130.42 mg kg-1. zinc Sorbed zinc fraction varied significantly due to treatments and higher sorbed zinc content was recorded in treatment T9 (T3 + FS of Zn EDTA @ 1.00 %) which was 3.89 mg kg1 which was significantly higher than treatments T1i.eNPK + FYM (1.87 mg kg-1), T4 (1.79 mg kg-1) and T5 (1.84 mg kg-1) and on par with rest of the treatments (T2, T3, T6, T7, T8). Easily reducible manganese bound zinc The reducible manganese bound zinc content in soil differed significantly due to treatment. Significantly higher easily reducible manganese bound fraction was observed in treatment T7 (4.2 mg kg-1) which received T3+ FS of Zn EDTA @ 0.50 per cent followed by T9 (4.18 mg kg-1) and were on par with treatment T2 (4.08 mg kg-1), T3 (4.14 mg kg-1),T6 (4.10 mg kg-1) and T8 (4.09 mg kg-1) and significantly higher than treatment T1i.e NPK + FYM (3.45 mg kg-1), T4 (3.43 mg kg-1) and T5 (3.47 mg kg-1). Distribution of zinc fractions in soil at harvest of greengram Sorbed water soluble zinc Significantly higher water soluble fraction was observed in treatment T7 (T3 + FS of Zn EDTA @ 0.5 %) (0.46 mg kg-1) followed by T9 (0.45 mg kg-1) and T3 (0.45 mg kg-1) and were significantly higher than rest of the treatments T1 NPK +FYM (0.32 mgkg-1), T2 510