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Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 466-468 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 10 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.710.050 Effect of GnRH on 5th Day of Post Artificial Insemination for Fertility Augmentation in Repeat Breeder Dairy Cows C.K. Lakde1*, S.K. Sahatpure1, S.K. Sheetal1, A.D. Patil1, S.V. Upadhye2, J.P. Korde3 and D.V. Patil4 1 Department of Animal Reproduction Gynaecology and Obstetrics, 2Department of Veterinary Surgery and Radiology, 3Department of Veterinary Physiology, 4Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Seminary Hills, Nagpur – 440 006 (Maharashtra), India *Corresponding author ABSTRACT Keywords Conception rate, Dairy cow, Fertility, GnRH, Non-infectious repeat breeder Article Info Accepted: 06 September 2018 Available Online: 10 October 2018 The objective of study to compare the conception rate in repeat breeder dairy cows after using injection of GnRH (Ovulanta)@ 20 μg intramuscularly on 5th day of artificial insemination (AI). Non-infectious cows were randomly divided into two groups Group I (no treatment) and Group II (GnRH on 5th day of post AI). At the time of AI, mean serum glucose concentration recorded 58 and 61 mg/dl in Group I and II. All the cows were screened for pregnancy diagnosis on day 45. The pregnancy percentage observed in Group I and Group II were 10 and 20 % respectively. From the study we concluded that the GnRH on 5th day of post AI was not enhancing the reproductive performance up to the mark in field condition. Introduction To treat reproductive disorder and to improve fertility in bovines a GnRH or its agonist has been used commonly in field condition. In dairy cows reproductive efficiency is not optimal, so for improving it with use of artificial insemination (AI) and conception rate some strategies must be focused. GnRH injection leads to LH secretion which causes leutinization and then progesterone secretion. So to prevent embryonic death due to luteal insufficiency GnRH treatment is beneficial (Sheldon and Dobson 1993). Injection of GnRH during mid-luteal phase after insemination induces sufficient release of LH and FSH to increase the life span of corpus luteum by counteracting luteolysis through disruption of normal follicular growth and secretion of estrogen, thereby permitting maternal recognition of pregnancy to occur (Willard et al., 2003). Materials and Methods The dairy cows were selected for the experiment maintained on individual dairy farms. Selection was strictly based on at least 466 Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 466-468 repeated by three AI attempt. At the time of AI white side test performed to identify whether cow was infectious or non-infectious repeat breeder. In the present experiment only non-infectious repeat breeder cow were selected. At the time of AI, blood was collected to estimate serum glucose concentration. Artificial insemination was performed as per AM-PM rule with only one in attempt. In Group I (n=10) no treatment was given to cow after AI and Group II (n=10) cows receive GnRH @ (20 μg intramuscularly) on 5th day of post AI. After 45 day of post AI, Ultrasonographical examination performed to screen out conception rate. Results and Discussion The dairy cows were selected for the experiment was strictly in non-infectious type. All cows were maintained on individual farmers place. Selection was strictly based on at least repeated by three AI attempt. Before start of experiment all cows were screened out for health check and to check any reproductive abnormality by ultrasonographical examination. All cows were supplemented with dewormed and supplemented with daily mineral mixture. At the time of AI mean blood serum glucose 58 and 61 mg/dl in group I and II recorded respectively. The findings are similar to Guzel and Tanriverdi (2014) where he reported blood glucose level in repeat breeder cow was 65.00±6.27 mg/dL. In group I, no treatment was given to cows who receive AI before 5 days and in Group II injection GnRH (Inj. Ovulata 20µg) administered intramuscularly. After 45 days of AI, pregnancy diagnosis was carried out. In the present experiment, in Group I (1 out of 10) 10% and in Group II (2 out of 10) 20% pregnancy recorded. These findings were comparable with Howard et al., (2006), who compare the conception rates in dairy cattle administered gonadotropin releasing hormone 5 days after AI and recorded 26.7% conception rate and concluded no effect of treatment as conception rates did not differ between GnRH and saline groups (26.7% GnRH versus 24.3% saline).In another study, Chandra Prasad and Ananda Rao (2014) conducted trial on murraha buffaloes with administration of burselin acetate (GnRH) on different days after insemination and reported that 37.5% animals conceived with use of receptal 5ml (0.021mg) of on day 5 of oestrus cycle. While Ahmed et al., (2010) conducted study on repeat breeding buffaloes as clinical perspective with use of GnRH on day 5th of post AI and recorded 61.54% conception rate. So these finding not in accordance with present findings. Similar opinion of Arnett et al., (2002) reported that administration of GnRH on day 5 or 6 after estrous was found to alter follicular dynamics induce luteal tissue development and increase progesterone concentration up to day 13 resulting in increased pregnancy rate and with finding of Pandey et al., 2016 who recorded 50% of conception rate when injected GnRH at 10 μg intramuscularly on 5th day of AI. So these findings are not accordance with the present study. Table.1 Effect of GnRH injection at day 5 after AI and conception on repeat breeder cows Sr. No. 1 2 Groups Group I (n=10, No treatment) Group II (n=10, GnRH Treated) 467 Conception rate (1/10) 10% (2/10) 20% Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 466-468 The injection of GnRH on 5th day of post AI can improve the reproductive performance in field condition when compared to up to no treated animal but not up to the mark. Acetate on Different Days of Estrous Cycle in Graded Murraha Buffaloes. International Journal of Recent Scientific Research. 5(9), 1558-1559. Howard J.M., R. Manzo, J.C. Dalton, F. Frago and A. Ahmadzadeha (2006) Conception rates and serum progesterone concentration in dairy cattle administered gonadotropin releasing hormone 5 days after artificial insemination. Animal Reproduction Science 95 224–233. Pandey NKJ, Gupta HP, Prasad S, Sheetal SK (2016) Plasma progesterone profile and conception rate following exogenous supplementation of gonadotropinreleasing hormone, human chorionic gonadotropin, and progesterone releasing intra-vaginal device in repeatbreeder crossbred cows, Veterinary World, 9(6): 559-562. Sheldon, M. and Dobson, H. (1993) Effects of gonadotrophin releasing hormone administered 11 days after insemination on the pregnancy rates of cattle to the first and later services. Vet Rec 133: 160-163. Willard, S., Gandy, S., Bowers, S., Graves, K., Elias, A. and Whisnant, C. (2003) The effects of GnRH administration post insemination on serum concentrations of progesterone and pregnancy rates in dairy cattle exposed to mild summer heat stress. Theriogenology, 59: 1799-1810. Acknowledgement The authors wish to thank Associate Dean of Nagpur Veterinary College, Nagpur, Maharashtra, for permitting to carry out the research work and providing necessary facilities. The authors are especially thankful to the Dean, Maharashtra Animal and Fishery Sciences University, Nagpur for providing fund and necessary facilities in the Department of Animal reproduction, Gynecology and Obstetrics for the smooth progress of the PhD research work and for their encouragement to complete the work in time. References Ahmed W.M., El-khadrawy H.H., Emtenan M. H., Amal H.A. and Shalaby S.A. (2010). Clinical perspective of repeat breeding syndrome in buffaloes. J. Am. Sci. 6(11):661-666. Arnett, M., Rhiehart, J., Barley, J. D., Highstone, R. B. and Anderson, L. H. (2002) Administration of GNRH on day 5 or 6 of the estrous cycle alters follicle dynamics and increase pregnancy rate in beef cattle. Journal of Animal Science, 80:133-34. Chandra Prasad B. and K. Ananda Rao (2014) Effect of Administration of Burselin How to cite this article: Lakde, C.K., S.K. Sahatpure, S.K. Sheetal, A.D. Patil, S.V. Upadhye, J.P. Korde and Patil, D.V. 2018. Effect of GnRH on 5th Day of Post Artificial Insemination for Fertility Augmentation in Repeat Breeder Dairy Cows. Int.J.Curr.Microbiol.App.Sci. 7(10): 466-468. doi: https://doi.org/10.20546/ijcmas.2018.710.050 468